PhytophthoraV1, Main, Exploration, bibRecord, 001825

The 74-kilodalton immunodominant antigen of the pathogenic oomycete Pythium insidiosum is a putative exo-1,3-beta-glucanase.

Identifieur interne : 001825 ( Main/Exploration ); précédent : 001824; suivant : 001826

The 74-kilodalton immunodominant antigen of the pathogenic oomycete Pythium insidiosum is a putative exo-1,3-beta-glucanase.

Auteurs : Theerapong Krajaejun [Thaïlande] ; Angsana Keeratijarut ; Kanchana Sriwanichrak ; Tassanee Lowhnoo ; Thidarat Rujirawat ; Thanom Petchthong ; Wanta Yingyong ; Thareerat Kalambaheti ; Nat Smittipat ; Tada Juthayothin ; Thomas D. Sullivan

Source :

Clinical and vaccine immunology : CVI [ 1556-679X ] ; 2010.

RBID : pubmed:20237199

Descripteurs français

English descriptors

KwdEn :
- Algal Proteins (chemistry), Algal Proteins (genetics), Algal Proteins (immunology), Animals (MeSH), Cluster Analysis (MeSH), DNA Primers (genetics), DNA, Algal (chemistry), DNA, Algal (genetics), Genotype (MeSH), Glucan 1,3-beta-Glucosidase (chemistry), Glucan 1,3-beta-Glucosidase (genetics), Glucan 1,3-beta-Glucosidase (immunology), Glucan 1,3-beta-Glucosidase (metabolism), Humans (MeSH), Immunodominant Epitopes (chemistry), Immunodominant Epitopes (genetics), Immunodominant Epitopes (immunology), Mass Spectrometry (MeSH), Molecular Weight (MeSH), Phytophthora infestans (enzymology), Phytophthora infestans (genetics), Polymerase Chain Reaction (MeSH), Pythium (classification), Pythium (enzymology), Pythium (genetics), Pythium (immunology), Sequence Analysis, DNA (MeSH), Sequence Homology, Amino Acid (MeSH), Sequence Homology, Nucleic Acid (MeSH).
MESH :
- chemical , chemistry : Algal Proteins, DNA, Algal, Glucan 1,3-beta-Glucosidase, Immunodominant Epitopes.
- chemical , genetics : Algal Proteins, DNA Primers, DNA, Algal, Glucan 1,3-beta-Glucosidase, Immunodominant Epitopes.
- chemical , immunology : Algal Proteins, Glucan 1,3-beta-Glucosidase, Immunodominant Epitopes.
- chemical , metabolism : Glucan 1,3-beta-Glucosidase.
- classification : Pythium.
- enzymology : Phytophthora infestans, Pythium.
- genetics : Phytophthora infestans, Pythium.
- immunology : Pythium.
- Animals, Cluster Analysis, Genotype, Humans, Mass Spectrometry, Molecular Weight, Polymerase Chain Reaction, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Sequence Homology, Nucleic Acid.

Abstract

The oomycetous, fungus-like, aquatic organism Pythium insidiosum is the causative agent of pythiosis, a life-threatening infectious disease of humans and animals living in tropical and subtropical areas of the world. Common sites of infection are the arteries, eyes, cutaneous/subcutaneous tissues, and gastrointestinal tract. Diagnosis of pythiosis is time-consuming and difficult. Radical excision of the infected organs is the main treatment for pythiosis because conventional antifungal drugs are ineffective. An immunotherapeutic vaccine prepared from P. insidiosum crude extract showed limited efficacy in the treatment of pythiosis patients. Many pythiosis patients suffer lifelong disabilities or die from an advanced infection. Recently, we identified a 74-kDa major immunodominant antigen of P. insidiosum which could be a target for development of a more effective serodiagnostic test and vaccines. Mass spectrometric analysis identified two peptides of the 74-kDa antigen (s74-1 and s74-2) which perfectly matched a putative exo-1,3-ss-glucanase (EXO1) of Phytophthora infestans. Using degenerate primers derived from these peptides, a 1.1-kb product was produced by PCR, and its sequence was found to be homologous to that of the P. infestans exo-1,3-ss-glucanase gene, EXO1. Enzyme-linked immunosorbent assays targeting the s74-1 and s74-2 synthetic peptides demonstrated that the 74-kDa antigen was highly immunoreactive with pythiosis sera but not with control sera. Phylogenetic analysis using part of the 74-kDa protein-coding sequence divided 22 Thai isolates of P. insidiosum into two clades. Further characterization of the putative P. insidiosum glucanase could lead to new diagnostic tests and to antimicrobial agents and vaccines for the prevention and management of the serious and life-threatening disease of pythiosis.

DOI: 10.1128/CVI.00515-09
PubMed: 20237199
PubMed Central: PMC2916248

Affiliations:

Thaïlande

Links toward previous steps (curation, corpus...)

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Le document en format XML

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<term>Algal Proteins (genetics)</term>
<term>Algal Proteins (immunology)</term>
<term>Animals (MeSH)</term>
<term>Cluster Analysis (MeSH)</term>
<term>DNA Primers (genetics)</term>
<term>DNA, Algal (chemistry)</term>
<term>DNA, Algal (genetics)</term>
<term>Genotype (MeSH)</term>
<term>Glucan 1,3-beta-Glucosidase (chemistry)</term>
<term>Glucan 1,3-beta-Glucosidase (genetics)</term>
<term>Glucan 1,3-beta-Glucosidase (immunology)</term>
<term>Glucan 1,3-beta-Glucosidase (metabolism)</term>
<term>Humans (MeSH)</term>
<term>Immunodominant Epitopes (chemistry)</term>
<term>Immunodominant Epitopes (genetics)</term>
<term>Immunodominant Epitopes (immunology)</term>
<term>Mass Spectrometry (MeSH)</term>
<term>Molecular Weight (MeSH)</term>
<term>Phytophthora infestans (enzymology)</term>
<term>Phytophthora infestans (genetics)</term>
<term>Polymerase Chain Reaction (MeSH)</term>
<term>Pythium (classification)</term>
<term>Pythium (enzymology)</term>
<term>Pythium (genetics)</term>
<term>Pythium (immunology)</term>
<term>Sequence Analysis, DNA (MeSH)</term>
<term>Sequence Homology, Amino Acid (MeSH)</term>
<term>Sequence Homology, Nucleic Acid (MeSH)</term>
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<term>ADN des algues (génétique)</term>
<term>Amorces ADN (génétique)</term>
<term>Analyse de regroupements (MeSH)</term>
<term>Analyse de séquence d'ADN (MeSH)</term>
<term>Animaux (MeSH)</term>
<term>Glucan 1,3-beta-glucosidase (composition chimique)</term>
<term>Glucan 1,3-beta-glucosidase (génétique)</term>
<term>Glucan 1,3-beta-glucosidase (immunologie)</term>
<term>Glucan 1,3-beta-glucosidase (métabolisme)</term>
<term>Génotype (MeSH)</term>
<term>Humains (MeSH)</term>
<term>Masse moléculaire (MeSH)</term>
<term>Phytophthora infestans (enzymologie)</term>
<term>Phytophthora infestans (génétique)</term>
<term>Protéines d'algue (composition chimique)</term>
<term>Protéines d'algue (génétique)</term>
<term>Protéines d'algue (immunologie)</term>
<term>Pythium (classification)</term>
<term>Pythium (enzymologie)</term>
<term>Pythium (génétique)</term>
<term>Pythium (immunologie)</term>
<term>Réaction de polymérisation en chaîne (MeSH)</term>
<term>Similitude de séquences d'acides aminés (MeSH)</term>
<term>Similitude de séquences d'acides nucléiques (MeSH)</term>
<term>Spectrométrie de masse (MeSH)</term>
<term>Épitopes immunodominants (composition chimique)</term>
<term>Épitopes immunodominants (génétique)</term>
<term>Épitopes immunodominants (immunologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Algal Proteins</term>
<term>DNA, Algal</term>
<term>Glucan 1,3-beta-Glucosidase</term>
<term>Immunodominant Epitopes</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Algal Proteins</term>
<term>DNA Primers</term>
<term>DNA, Algal</term>
<term>Glucan 1,3-beta-Glucosidase</term>
<term>Immunodominant Epitopes</term>
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<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Algal Proteins</term>
<term>Glucan 1,3-beta-Glucosidase</term>
<term>Immunodominant Epitopes</term>
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<term>Glucan 1,3-beta-glucosidase</term>
<term>Protéines d'algue</term>
<term>Pythium</term>
<term>Épitopes immunodominants</term>
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<term>Pythium</term>
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<term>Pythium</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Phytophthora infestans</term>
<term>Pythium</term>
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<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN des algues</term>
<term>Amorces ADN</term>
<term>Glucan 1,3-beta-glucosidase</term>
<term>Phytophthora infestans</term>
<term>Protéines d'algue</term>
<term>Pythium</term>
<term>Épitopes immunodominants</term>
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<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Glucan 1,3-beta-glucosidase</term>
<term>Protéines d'algue</term>
<term>Pythium</term>
<term>Épitopes immunodominants</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Pythium</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glucan 1,3-beta-glucosidase</term>
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<term>Cluster Analysis</term>
<term>Genotype</term>
<term>Humans</term>
<term>Mass Spectrometry</term>
<term>Molecular Weight</term>
<term>Polymerase Chain Reaction</term>
<term>Sequence Analysis, DNA</term>
<term>Sequence Homology, Amino Acid</term>
<term>Sequence Homology, Nucleic Acid</term>
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<keywords scheme="MESH" xml:lang="fr"><term>Analyse de regroupements</term>
<term>Analyse de séquence d'ADN</term>
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<term>Génotype</term>
<term>Humains</term>
<term>Masse moléculaire</term>
<term>Réaction de polymérisation en chaîne</term>
<term>Similitude de séquences d'acides aminés</term>
<term>Similitude de séquences d'acides nucléiques</term>
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<front><div type="abstract" xml:lang="en">The oomycetous, fungus-like, aquatic organism Pythium insidiosum is the causative agent of pythiosis, a life-threatening infectious disease of humans and animals living in tropical and subtropical areas of the world. Common sites of infection are the arteries, eyes, cutaneous/subcutaneous tissues, and gastrointestinal tract. Diagnosis of pythiosis is time-consuming and difficult. Radical excision of the infected organs is the main treatment for pythiosis because conventional antifungal drugs are ineffective. An immunotherapeutic vaccine prepared from P. insidiosum crude extract showed limited efficacy in the treatment of pythiosis patients. Many pythiosis patients suffer lifelong disabilities or die from an advanced infection. Recently, we identified a 74-kDa major immunodominant antigen of P. insidiosum which could be a target for development of a more effective serodiagnostic test and vaccines. Mass spectrometric analysis identified two peptides of the 74-kDa antigen (s74-1 and s74-2) which perfectly matched a putative exo-1,3-ss-glucanase (EXO1) of Phytophthora infestans. Using degenerate primers derived from these peptides, a 1.1-kb product was produced by PCR, and its sequence was found to be homologous to that of the P. infestans exo-1,3-ss-glucanase gene, EXO1. Enzyme-linked immunosorbent assays targeting the s74-1 and s74-2 synthetic peptides demonstrated that the 74-kDa antigen was highly immunoreactive with pythiosis sera but not with control sera. Phylogenetic analysis using part of the 74-kDa protein-coding sequence divided 22 Thai isolates of P. insidiosum into two clades. Further characterization of the putative P. insidiosum glucanase could lead to new diagnostic tests and to antimicrobial agents and vaccines for the prevention and management of the serious and life-threatening disease of pythiosis.</div>
</front>
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<Abstract><AbstractText>The oomycetous, fungus-like, aquatic organism Pythium insidiosum is the causative agent of pythiosis, a life-threatening infectious disease of humans and animals living in tropical and subtropical areas of the world. Common sites of infection are the arteries, eyes, cutaneous/subcutaneous tissues, and gastrointestinal tract. Diagnosis of pythiosis is time-consuming and difficult. Radical excision of the infected organs is the main treatment for pythiosis because conventional antifungal drugs are ineffective. An immunotherapeutic vaccine prepared from P. insidiosum crude extract showed limited efficacy in the treatment of pythiosis patients. Many pythiosis patients suffer lifelong disabilities or die from an advanced infection. Recently, we identified a 74-kDa major immunodominant antigen of P. insidiosum which could be a target for development of a more effective serodiagnostic test and vaccines. Mass spectrometric analysis identified two peptides of the 74-kDa antigen (s74-1 and s74-2) which perfectly matched a putative exo-1,3-ss-glucanase (EXO1) of Phytophthora infestans. Using degenerate primers derived from these peptides, a 1.1-kb product was produced by PCR, and its sequence was found to be homologous to that of the P. infestans exo-1,3-ss-glucanase gene, EXO1. Enzyme-linked immunosorbent assays targeting the s74-1 and s74-2 synthetic peptides demonstrated that the 74-kDa antigen was highly immunoreactive with pythiosis sera but not with control sera. Phylogenetic analysis using part of the 74-kDa protein-coding sequence divided 22 Thai isolates of P. insidiosum into two clades. Further characterization of the putative P. insidiosum glucanase could lead to new diagnostic tests and to antimicrobial agents and vaccines for the prevention and management of the serious and life-threatening disease of pythiosis.</AbstractText>
</Abstract>
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<Initials>T</Initials>
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<Initials>T</Initials>
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<Initials>T</Initials>
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<ForeName>Nat</ForeName>
<Initials>N</Initials>
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<ForeName>Tada</ForeName>
<Initials>T</Initials>
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<Author ValidYN="Y"><LastName>Sullivan</LastName>
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<ArticleId IdType="pii">CVI.00515-09</ArticleId>
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<ArticleId IdType="pmc">PMC2916248</ArticleId>
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<ReferenceList><Reference><Citation>Infect Immun. 2000 Dec;68(12):6777-84</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11083795</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Clin Diagn Lab Immunol. 2002 Mar;9(2):378-82</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11874882</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Vet Intern Med. 2002 Mar-Apr;16(2):142-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11899028</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Vet Intern Med. 2002 Mar-Apr;16(2):147-52</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11899029</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Fungal Genet Biol. 2003 Mar;38(2):250-63</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12620261</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Eukaryot Cell. 2003 Apr;2(2):191-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12684368</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Vaccine. 2003 Jun 2;21(19-20):2535-40</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12744888</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mycol Res. 2003 May;107(Pt 5):537-44</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12884950</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Indian J Pediatr. 2004 Mar;71(3):253-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15080413</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Clin Microbiol. 2004 Jun;42(6):2411-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15184412</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Microbiology. 2004 Jul;150(Pt 7):2029-35</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15256547</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Vaccine. 2004 Sep 9;22(27-28):3613-21</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15315840</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Clin Microbiol. 2004 Sep;42(9):3970-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15364977</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Med Mycol. 2005 Sep;43(6):477-86</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16320491</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Clin Microbiol. 2006 May;44(5):1674-80</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16672392</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Trends Ecol Evol. 2005 Dec;20(12):670-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16701456</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Clin Infect Dis. 2006 Sep 1;43(5):569-76</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16886148</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Microbiol. 2007 May;9(5):1223-38</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17217426</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>FEMS Microbiol Rev. 2007 Mar;31(2):168-92</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17313520</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Southeast Asian J Trop Med Public Health. 2007 Mar;38(2):383-91</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17539291</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Clin Microbiol. 1991 Nov;29(11):2661-2</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1774283</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Antimicrob Chemother. 2007 Nov;60(5):1168-71</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17785281</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Med Mycol. 2008 Feb;46(1):41-52</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17885956</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2008 Mar 05;3(3):e1723</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18320043</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Am J Vet Res. 2008 Nov;69(11):1463-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18980428</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Clin Vaccine Immunol. 2009 Apr;16(4):506-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19225072</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Clin Vaccine Immunol. 2009 Jul;16(7):1047-51</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19494087</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Med Assoc Thai. 2009 Aug;92(8):1063-71</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19694332</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Southeast Asian J Trop Med Public Health. 2009 Nov;40(6):1298-305</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20578465</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mycoses. 1990 Jun;33(6):317-23</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2259373</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Evolution. 1985 Jul;39(4):783-791</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28561359</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Am J Vet Res. 1988 Nov;49(11):1866-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3073678</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Clin Microbiol. 1986 May;23(5):813-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3086368</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mycopathologia. 1988 Oct;104(1):59-62</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3216883</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Bacteriol. 1966 Mar;91(3):1080-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">5929744</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Enzyme Microb Technol. 1993 Mar;15(3):178-92</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7763458</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Clin Infect Dis. 1994 Aug;19 Suppl 1:S1-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7948565</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 1994 Nov 11;22(22):4673-80</Citation>
<ArticleIdList><ArticleId IdType="pubmed">7984417</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Clin Microbiol. 1993 Nov;31(11):2967-73</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8263182</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Comput Appl Biosci. 1996 Aug;12(4):357-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8902363</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Clin Diagn Lab Immunol. 1997 Nov;4(6):715-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9384295</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
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The 74-kilodalton immunodominant antigen of the pathogenic oomycete Pythium insidiosum is a putative exo-1,3-beta-glucanase.

The 74-kilodalton immunodominant antigen of the pathogenic oomycete Pythium insidiosum is a putative exo-1,3-beta-glucanase.

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